Human fibroblasts have been collected from volunteers participating in the clinical sample healthy donor cohort of the Milieu Intérieur project. Skin biopsies were taken from the internal part of the forearm. Primary fibroblasts isolation was performed at Genethon (Evry, France) using the explant culture protocol. Each derived primary cell isolate is about 10 million cells frozen at passage 3.
The objectives are to:
- Establish a unique biobank of fibroblasts from the Milieu Intérieur cohort with deep annotation of healthy human individuals
- Establish novel approaches using cell-based screening assays and statistical tools to better characterize variability in innate immune response, infectious processes, and cellular mechanisms underlying inflammation and cancer
- Propagate the collection using immortalization and/or iPS approaches that preserve the epidemiological characteristics, and diversity of response patterns. The main value of this collection lies in the distribution of the donors’ cell response as a surrogate of the variability of the responses in a population, with the aim of reproducing this variability experimentally in in vitro tests. To preserve this value, we developed a "controlled immortalization" strategy.